ncbi genome sequencing bioproject  (ATCC)

Journal: Cell reports

Article Title: A Two-Enzyme Adaptive Unit within Bacterial Folate Metabolism

doi: 10.1016/j.celrep.2019.05.030

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli K12 MG1655 CGSC CGSC#:7740 MG1655 with chromosomal dcas9 Gift from David Bikard N/A ER2566 ΔfolA ΔthyA Gift from S. Benkovic N/A Chemicals, Peptides, and Recombinant Proteins Purezol Bio-Rad Cat#7326890 Amicase Sigma Cat#65072-00-6 MOPS EZ Rich Defined Medium Teknova Cat#M2105 Critical Commercial Assays 300 cycle Illumina MiSeq kit V2 Illumina Cat#MS-102-2002 Luna Universal One-Step RT-qPCR kit NEB Cat#E3005S QIAamp DNA Minit Kit QIAGEN Cat#51304 Nextera XT DNA Library Prep kit Illumina Cat#FC-131-1096 50 cycle Illumina MiSeq kit V2 Illumina Cat#MS-102-2001 Deposited Data Evolved MG1655 genome sequencing reads NCBI BioProject PRJNA378892 Experimental Models: Organisms/Strains MG1655 with chromosomal dcas9 and sgRNA library (gAM-350) This study N/A Evolved MG1655 strains (5thy condition) This study N/A Evolved MG1655 strains (10thy condition) This study N/A Evolved MG1655 strains (50thy condition) This study N/A MG1655 reconstitution strains This study N/A MG1655 folA single mutants Gift from E. Toprak ( Palmer et al., 2015 ) N/A ER2566 ΔfolA ΔthyA pACYC folA/thyA library This study N/A Oligonucleotides Table S2 : Primers used for CRISPRi and qPCR This study N/A Recombinant DNA pAM-111 This study N/A pAM-112 This study N/A pAM-288/290 This study N/A pCRISPR Jiang et al., 2013 Addgene plasmid #42875 pgRNA Qi et al., 2013 Addgene plasmid #44251 pTKRED Kuhlman and Cox.

Techniques: Recombinant, Sequencing, Plasmid Preparation, Software